Natural variability in the Arabidopsis response to infection with Erwinia carotovora subsp. carotovora
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چکیده
منابع مشابه
Efficient transformation of Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica.
We used a modified version of the method of Hanahan (D. Hanahan, J. Mol. Biol. 166:557-580, 1983) to transform Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica with the plasmids pBR322, pBR325, and pAT153. The transformation frequency ranged from 1 X 10(2) to 4 X 10(4) colonies per micrograms of plasmid DNA. The nature of these transformants was confirmed by plasmid ana...
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The term endophyte refers to interior colonization of plants by microorganisms that do not have pathogenic effects on their hosts, and various endophytes have been found to play important roles in plant vitality. In this study, cultivation-independent terminal restriction fragment length polymorphism analysis of 16S ribosomal DNA directly amplified from plant tissue DNA was used in combination ...
متن کاملRegulation of the expression of prtW::gusA fusions in Erwinia carotovora subsp. carotovora.
Erwinia carotovora subsp. carotovora, a Gram-negative phytopathogenic bacterium, secretes an extracellular metalloprotease, PrtW. Previous results demonstrated that protease activity is necessary for the normal progression of disease symptoms caused by this bacterium. The present study revealed that the prtW gene constitutes an independent transcriptional unit. It is demonstrated that introduct...
متن کاملGenomic diversity of Erwinia carotovora subsp. carotovora and its correlation with virulence.
We used genetic and biochemical methods to examine the genomic diversity of the enterobacterial plant pathogen Erwinia carotovora subsp. carotovora. The results obtained with each method showed that E. carotovora subsp. carotovora strains isolated from one ecological niche, potato plants, are surprisingly diverse compared to related pathogens. A comparison of 23 partial mdh sequences revealed a...
متن کاملQuorum sensing in the plant pathogen Erwinia carotovora subsp. carotovora: the role of expR(Ecc).
The production of the main virulence determinants of the plant pathogen Erwinia carotovora subsp. carotovora, the extracellular cell wall-degrading enzymes, is partly controlled by the diffusible signal molecule N-(3-oxohexanoyl)-L-homoserine lactone (OHHL). OHHL is synthesized by the product of the expI/carI gene. Linked to expI we found a gene encoding a putative transcriptional regulator of ...
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ژورنال
عنوان ژورنال: Planta
سال: 2002
ISSN: 0032-0935,1432-2048
DOI: 10.1007/s00425-002-0743-3